Part:BBa_K2649000
ComE-NLS-VP16-NES S. mutans SM6
This part contains a human optimized coding sequence for ComE, the putative response regulator protein for the ComCDE two-component signaling pathway, native to Streptococcus mutans strain SM6. Design elements were chosen to optimize expression and function of ComE in human cells, as part of an attempt to port functioning two-component ComCDE signalling to mammalian cells. To be used as part of a signaling system, the ComE response regulator must be paired with the ComD histidine kinase receptor protein. When ComD binds to the S. mutans competence stimulating peptide, it autophosphorylates and transfers a phosphate group to an aspartic acid on ComE, enabling ComE to oligomerize and bind to specific DNA sequences to promote transcription. The ComE coding sequence is fused to a nuclear localization sequence (NLS), VP16 activating domain, and a nuclear export sequence (NES). It is in a pSB1C3 backbone and is BioBrick and MoClo compatable.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
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